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Description
Human PIICP ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. 3. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Procollagen II C-Terminal Propeptide (PIICP). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Procollagen II C-Terminal Propeptide (PIICP) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Procollagen II C-Terminal Propeptide ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Type II procollagen carboxyl-terminal peptide (PⅡCP), also known as CP-II or P2CP, is used as a biomarker to investigate cartilage damage and repair in rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, and in humans and several animal models. The concentration of CP-II in cartilage is directly correlated with the synthesis of type II collagen. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.8 ★★★★★
Based on 2314 reviews
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Product Reviews
★★★★★ 5
Accurate reviews for fit n color
Size: 32, Color: Brown
I’m about a 33 waist I got a 32 of other reviews and recommendations. Do you run slightly large so it fits great Color slightly off, but I also do that in other review. Purchase two.
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Reviewed in the United States on March 2, 2026
★★★★★ 5
“Elegant, Practical, and the Perfect Gift .
Color: Black Cambridge
It’s one of those pieces that feels both classic and modern at the same time, perfect for any occasion.
The removable ID window is a very useful feature, especially for someone who needs to show their ID frequently.
The interior design is also very well thought out—everything stays organized and easy to access. I like that it combines functionality with style, which isn’t always easy to find. It definitely feels like a durable and well-made product.
It’s a gift that truly feels worth it and special. I would definitely recommend it to anyone looking for a stylish, practical, and high-quality wallet.
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Reviewed in the United States on March 17, 2026
★★★★★ 5
If you're looking for a high-capacity wallet, look no further!
Here's the breakdown on slots: It's a bifold with a passcase kind of flap. Standard bill holder with no divider. On the left, it has 3 slots to hold cards horizontally, and 2 slots to hold cards vertically, for a total of 5 slots. Right is the same design, 5 slots. The passcase-like flap has a clear cutout for an ID on the left side, and on the right side, it has 3 slots to hold cards vertically. We're up to 13 slots. There are also 3 pockets in the wallet when the passcase-like flap is moved to the left side of the wallet: One on the right behind the credit cards, one on the left behind the credit cards, and one in the passcase-like flap under the flap's credit cards. That's 13 cards, and 3 pockets that can store insurance cards, business cards, or even 3 or 4 stacked credit cards in each flap. One could theoretically cram 25 credit cards in this wallet. Realistically though, this will hold 13 cards, as those pockets will likely be holding other personal effects.
As for the quality, it's pretty great. The material is leather, and the cloth in the bill holder is diagonally-striped burgundy and navy cloth. In the middle of the wallet, with the pass-case like flap to the left, there is vertically-striped cloth; the color is burgundy surrounding one stripe of navy, with an ivory border between them. It looks very well-made. I also absolutely that some card slots are horizontal and some are vertical, as I can organize my cards in groups (ie: my 2 credit cards from one bank go vertically on left, my 5 from another hog the entire right side, my 3 debit cards rest in the passcase-like flap's card slots). In terms of being able to organize stuff, this wallet is a masterpiece. An absolute, ultimate, card-hungry masterpiece. I've had other wallets with less slots and had to resort to double-stacking credit cards into slots in order to carry them, which only led to premature wear and faster replacement of those wallets (not to mention the slots getting stretched to the point that cards were no longer secure and would fall out if the wallet was opened and shook). This is why I am writing such a raving review. For anyone that has many credit cards, those FICO score hypermiler types (as for myself, I'm up to 7 credit cards now), this wallet is a masterpiece.
With 13 cards, 2 IDs resting in the clear window on the passcase-like flap, about 10 bills, and about 5 paper cards in the pockets, my wallet measures about 1.25" thick at the thickest point... It might even be 1.5" thick, as I'm just making a visual estimation. It's a fat, fat wallet, but only because it's so stuffed with things I otherwise couldn't carry if I resorted to a less spacious wallet. I normally would be bugged by this lump that is noticeably larger than other wallets, but 2 days into owning this, I find it kind of reassuring, as I know this puppy is full of every credit card, bank card, ID, and insurance card I own. It feels good to sit down and think that I can fit every card I own in it perfectly. This wallet is for people who have a lot to carry and want to carry it all. If the desire to carry everything outweighs the inconvenience of the wallet's width, it's a great wallet.
When it comes to wallets, there are two people in this world: those that don't need this many card slots and would dislike the thickness of this wallet; and people who could use most of these slots and would appreciate being able to do so, when other wallets leave them wanting for space. I'd recommend this for the latter, and for the former, I'd recommend getting a wallet with less capacity and less size. For the latter, I think your search ends right here. It's such a good wallet for capacity that I just wrote 3 pages on it.
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Reviewed in the United States on October 5, 2016
★★★★★ 5
Good Quality
Color: Blue/White/Red
I bought it for my boyfriend
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Reviewed in the United States on May 14, 2026
★★★★★ 5
Tommy Hilfiger Slim Minimalist Wallet for Men - A Perfect Blend of Style and Functionality
Color: True Black, Color: True Black
I recently purchased the Tommy Hilfiger Slim Minimalist Wallet for men, and I couldn’t be happier with this product! This wallet is everything I was looking for: sleek, stylish, and incredibly functional.
First and foremost, it lives up to its name—absolutely slim. It slides effortlessly into my pocket without adding unnecessary bulk, making it perfect for everyday carry or formal occasions. Despite its compact size, it offers just the right amount of space for my essentials, including a few cards and some cash.
The craftsmanship is top-notch, as expected from Tommy Hilfiger. The minimalist design paired with the brand’s subtle logo adds a touch of elegance and class. The materials feel premium, and I’m confident it will last me for years.
What I love most is how practical it is without compromising on style. It’s lightweight yet sturdy, making it a great option for those who prefer a fuss-free wallet that still makes a statement.
Overall, I highly recommend this wallet to anyone in need of a compact, durable, and fashionable accessory. It’s truly an excellent addition to my everyday essentials.
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Reviewed in the United States on January 10, 2025