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Description
Human TINF2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a TERF1 Interacting Nuclear Factor 2 (TINF2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of TERF1 Interacting Nuclear Factor 2 (TINF2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human TERF1 Interacting Nuclear Factor 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | TERF1-interacting nuclear factor 2 (TINF2), also known as TRF1-interacting nuclear protein 2 (TIN2), is a protein encoded by the TINF2 gene. It is a component of the shelterin protein complex found at the ends of telomeres. This gene encodes one of the proteins of the shelterin or telosome complex, which protects telomeres by allowing cells to distinguish telomeres from areas of DNA damage. The protein encoded by this gene is a key component of shelterin. It interacts with the three DNA-binding proteins of the shelterin complex and is important for the assembly of the complex. Mutations in this gene cause dyskeratosis congenita (DKC), an inherited bone marrow failure syndrome. It has been shown to interact with ACD, POT1, and TERF1. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.6 ★★★★★
Based on 546 reviews
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Product Reviews
★★★★★ 5
Screamo for Creamo! Update on Sage and Citrus and Cologne
Scent: Bourbon Oak, Size: 16 Fl Oz (Pack of 2)
So first off the product came exactly as depicted. The smell was good but not strong or offensive. It has a pleasing woodsy smell. The name says it all. The lather is not particularly big but that doesn’t define how clean you get. I think it cleaned me up very well with one caveat. My face is a little oily so I did purchase the Creamo face wash. The body wash really didn’t do the trick for my face which is somewhat oily. The bottle is reminiscent of the past and stylish. It overall is a great product.
I just bought the Sage and Citrus body wash. This is a great all season body wash with a more contemporary smell. It’s lighter than the Bourbon and Oak with, as stated, a citrus smell tempered with the earthy sage. Again it does not clean my oily face but I use the Creamo face wash and it does just fine. The rest of my body feels clean and the scent is light and not overpowering. Between both products I also use the shaving cream.
I enjoy the original scent. I tried the Bourbon scent with the Bourbon and Oak body wash and felt it was a little overpowering. On a good note I combined all this with the Bourbon and Oak cologne. The shaving cream last a long time and should be used sparingly. The value is in the amount you use. It’s far less than a cheaper shaving cream but offers superior protection and excellent moisturizing elements.
The cologne lasts all day and does not require but a single spray under both arms and a single spray on a wrist rubbed against the other. If you need an anti perspirant use one without a scent since this will conflict with the cologne. The value is definitely there as this cologne albeit simple in appearance has very delicate notes reminiscent of my more expensive cologne. I use Creed Vetiver for going out. This cologne has a very similar quality but is, of course, a different bouquet.
Overall the whole line of Creamo products seems to be well thought out and outshines many higher end products such as Clinique or Brickell which I have also used. It comes down to value and quality. That’s where it really wins. It’s made in California where I live!
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Reviewed in the United States on August 23, 2019
★★★★★ 5
Smooth Lather and a Balanced, Refreshing Scent
Scent: Sage & Citrus, Size: 32 Fl Oz (Pack of 1)
This body wash delivers a rich, consistent lather and a scent that’s both clean and uplifting. The sage adds a subtle herbal note while the citrus keeps it bright. It rinses off without residue and doesn’t dry out the skin. A well‑crafted formula that feels premium without the premium price tag.
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Reviewed in the United States on February 26, 2026
★★★★★ 4
Does this beat Anthony Invigorating wash in the long last smell
Scent: Seagrass & Driftwood, Size: 32 Fl Oz (Pack of 1)
Anthony Invigorating wash (check out my review of that) has a very strong pine scent to it and is a very long lasting smell. How does Cremo's products stand up to it!
I put deodorants, shampoos, and body washes through the same rigorous tests. I run 14+ miles 4-5 times a week, swim 20+ lengths (or laps) in a junior Olympic pool 3 days a week, use a Sauna 2-3 times in fifteen minute intervals 4 times a week, and use weights 4 days a week. All of these body washes and shampoos that I try out not only go up against Anthony's but also getting rid of that chlorine smell. I always will finish a product before reviewing it
I'll be honest here as always, the Blue Cedar and Cypress at first whiff smelled weird. I can't explain the 1st whiff smell though you have my warning. I thought this was going to be my least favorite of the 3 different Cremo products (bourbon and oak, seagrass and driftwood, and this one) that I received. I have the policy to use all the products up in their entirety, so you don't have to. After using it up, I actually like the smell of it. The cedar smell comes out once applied. I actually use this with Anthony Blue Sea Kelp Body (check out my review of that). This goes great with it.
So on my buy, borrow, or bury review rating system, this is a buy. The issue I have with it, is that Cremo products don't seem to be that moisturizing their smells are good and lasting. This beats the off the chlorine smell of the pool and works great in combination with Anthony's. Try it out, just get past that initial smell, you won't be disappointed.
Now for Cremo's seagrass and driftwood. The First smell test of it is very pleasant. It's once you've put it on does that wood smell really come out. The smell is good and does last long though the issue I have that it is not moisturizing. This goes great with Anthony's other 2 products that I have viewed (please check those out). So on my buy, borrow, or bury rating scale, this is a buy. If they could make their products more moisturizing it would be fantastic.
I'll be back here to review the Bourbon and Oak, I have not finished it yet though it's probably going to be my favorite of all the 3 Cremo products I've tried out.
Please check out all of my other reviews!
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Reviewed in the United States on February 20, 2020
★★★★★ 5
Great buy!
Scent: Palo Santo, Size: 16 Fl Oz (Pack of 2)
This is my favorite brand and the lather is second to none! The smell is amazing and the quality is worth the extra price. It leaves my skin feeling moisturized and soft.
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Reviewed in the United States on March 4, 2026
★★★★★ 5
FINALLY. THIS IS ACTUALLY WHAT YOU WANT.
Scent: Seagrass & Driftwood, Size: 32 Fl Oz (Pack of 1)
I’ve tried all of the soaps. All of them. Molten brown sucks, 18.21 sucks, Blackwood sucks, and brickell sucks.
All I wanted is a premium soap that doesn’t suck. All I wanted is to feel good and smell good. I work in kitchens, so I’m not some desk monkey needing to wash off the stale air, I have to actually clean myself.
I don’t even know how these soaps stay in business, you might as well be using Irish spring and save the money. Nothing moisturizes, I’m always left with dry skin, or the smell lasts all of 6 minutes.
Until now.
This stuff is actually the luxury brand I’m swearing by. It lasts on my skin, it lathers well, it cleans well, and it doesn’t give me itchy skin just to smell nice after a shower.
Also, it doesn’t cost $36 per bottle like molten brown does for 2 teaspoons of soap with gold flakes in it that smells great for 6 minutes. It’s cheaper, and you get more.
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Reviewed in the United States on September 14, 2024