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Description
Human JAZF1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Juxtaposed With Another Zinc Finger Protein 1 (JAZF1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Juxtaposed With Another Zinc Finger Protein 1 (JAZF1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Juxtaposed With Another Zinc Finger Protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Juxtaposed zinc finger protein 1 (JAZF1), also known as TAK1-interacting protein 27 (TIP27) or zinc finger protein 802 (ZNF802), is a protein encoded by the JAZF1 gene. Variants are associated with an increased risk of prostate cancer, type 2 diabetes, and increased height. This gene encodes a nuclear protein with three C2H2-type zinc fingers and functions as a transcriptional repressor. Chromosomal aberrations involving this gene are associated with endometrial stromal tumors. Alternative splice variants encoding different protein isoforms have been described, however, not all variants have been fully characterized. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.6 ★★★★★
Based on 1446 reviews
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Product Reviews
★★★★★ 4
Video output does not contain power — caveats and findings for Switch users to whom this may concern
The product works with some caveats, which I initially tried to resolve with an ultimately unsuccessful firmware upgrade. (Anker was responsive via email, much appreciated.) It turns out the product was working to the best of its abilities this whole time, which I will now expound upon.
I expect that perhaps another 4-5 people in the world intended to use this in the ways that I did, namely with a Nintendo Switch as the source to various output devices. If you are one of these people who are interested in reading my experience connecting this hub with a Nintendo Switch, my findings are below (after a brief primer).
A Brief Primer
1. As you know, the Nintendo Switch can be coaxed to output Dp-Alt video if you can satisify a 39W+ handshake (15V, 2.6A or higher)
2. There are other adapters that will do this and output the video (with ample power) over USB-C, which are useful for powering AR glasses, portable monitors, or desktop USB-C monitors.
3. The limitation of a USB-C Dp-Alt adapter is that it does not also provide USB data ports like the Switch Dock itself, while many USB-C to HDMI dongles include USB-A ports. (But we don't want HDMI out, of course, because using USB-C we can power AR glasses or a portable monitor and send the video signal with one cable.)
4. I surmised that, by chaining a USB-C Dp Alt Adapter to this one (Anker A8340), I could have video output and three USB-C ports. Why do I want USB-C data ports? Since you are like me, you already know it is so I can connect a UAC1 DAC to output USB Audio and power thirsty headphones via a 4.4mm Pentacon balanced headphone jack. I also don't need to convince you of the merits of this versus using the Switch's built in 3.5mm headphone jack. Or say, connecting a wired controller because I don't care for input latency if I can avoid it.
My Findings
1. This hub can indeed be chained to a USB-C Dp-Alt adapter and pass through a video signal while also leaving three USB 3.2 data ports for wired devices (yes!)
2. However, as other reviews kindly mention, the video signal outputted by the A8340 does not contain power of any sort. So, if you are intending to plug this into a desktop USB-C monitor which already has/doesn't need power, then everything is awesome.
3. If you are trying to power AR glasses, this won't work unless you add a second USB-C DP-Alt adapter after it, and provide power that way. If you intend to convert the video signal to HDMI or DisplayPort via a USB-C to HDMI or DP cable, these won't work because there is no power for the inline conversion.
4. If you are using a USB-C travel monitor, this will work provided you directly power the monitor via its second USB-C power (if it has one).
So in summation, it's possible to get what you want, though you may find the victory pyrrhic. The perfect adapter (which I had hope this was, and may yet exist at a higher price point) basically takes a USB-C Dp-Alt adapter, adds USB-C data ports, and outputs USB-C + power to power AR glasses or a travel monitor.
This device nevertheless has its uses, such as the ability to connect multiple USB-C devices to the Switch in handheld mode and charge it, too.
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Reviewed in the United States on January 30, 2025
★★★★★ 5
Simple and reliable. No Flaws.
Flawless performance so far. I use this item with my iMac, and I'm able to run a 12 channel music interface through it with no noticeable lag.
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Reviewed in the United States on February 27, 2026
★★★★★ 5
Essential USB-C Adapters That Just Work Perfectly
These adapters are an absolute must-have and make life significantly easier when dealing with modern USB-C devices.
The fit is perfect, the adapters feel high quality, and they work flawlessly. Data transfer has been fast and reliable, and I have had no connectivity issues at all.
They are small enough to keep permanently attached to cables or stored in a laptop bag, making them incredibly convenient for everyday use.
As someone who regularly works with laptops, electronics, and multiple USB standards, I consider these essential accessories. They offer excellent value for the money and solve a surprisingly common problem very elegantly.
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Reviewed in the United States on May 27, 2026
★★★★★ 5
Works perfectly with MacBook
Bought these adapters after getting a wireless mouse for a MacBook and realizing the USB receiver couldn’t be connected directly because the Mac doesn’t have a regular USB port. These adapters solved the problem completely.
They work exactly as expected and were very easy to use. The quality feels good and the color matches the MacBook nicely, so they don’t look cheap or out of place. Small, simple, and stylish adapters that do their job well
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Reviewed in the United States on May 16, 2026
★★★★★ 5
Anker adapters
These Anker adapters honestly saved me. The quality feels very premium and they work perfectly with no disconnecting or recognition issues. I used them to connect USB-C accessories and everything worked instantly and smoothly.
They’re compact, durable, and super convenient to carry around for my content creation setup. The metallic finish also feels much more reliable compared to cheaper adapters I’ve tried before.
Definitely recommend this brand if you want something dependable and high quality for your devices. ✨
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Reviewed in the United States on May 25, 2026