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Description
Mouse ACAT1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an acetyl-CoA acetyltransferase, mitochondrial (ACAT1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of acetyl-CoA acetyltransferase, mitochondrial (ACAT1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Acetyl-CoA acetyltransferase, mitochondrial ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Acetyl-CoA acetyltransferase 1 (ACAT1), also known as acetoacetyl-CoA thiolase (ACAT), is an enzyme encoded by the ACAT1 gene. It is an acetyl-CoA C-acetyltransferase. This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetyl-CoA from two molecules of acetyl-CoA. The ACAT1 enzyme has several unique properties. First, it is activated by potassium ion binding near the CoA binding site and the catalytic site. This binding results in structural changes in the active site loop. Furthermore, the enzyme can use 2-methyl-branched acetoacetyl-CoA as a substrate, making it a unique thiolase. It is regulated at both the transcriptional and translational levels. Enhanced ACAT1 enzyme activity is transcriptionally promoted by leptin, angiotensin II, and insulin in human monocytes/macrophages. Mutations in this gene are associated with a deficiency of the encoded mitochondrial acetoacetyl-CoA thiolase protein, also known as ketothiolase deficiency. Its expression has been associated with the manifestation of prostate cancer. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.7 ★★★★★
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Product Reviews
★★★★★ 5
Sturdy, Extremely Adjustable, and Saves Huge Desk Space!
Size: 17"-49", Style: Single Arm
This WALI monitor mount is incredibly solid and handles the weight of my monitor easily without any sagging or shaking. The gas spring arm moves fluidly, making it super easy to adjust the height, angle, and tilt whenever I need to. Installation was straightforward with clear instructions, and it has freed up so much valuable desk space. Excellent build quality for a great price!
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Reviewed in the United States on May 29, 2026
★★★★★ 5
Solid Mount for a Large Monitor
Size: 17"-49", Style: Single Arm
Picked this up to mount a single 37" monitor to my adjustable desk and it’s been working great. The arm feels sturdy and holds the weight of the monitor without sagging or drifting over time.
Installation was pretty straightforward and it gave me a lot more desk space compared to using the factory stand. It also moves smoothly when adjusting the monitor position or changing desk height.
If you’re running a larger display on a standing desk setup, this is a solid option that feels much more substantial than some of the cheaper monitor arms out there.
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Reviewed in the United States on May 11, 2026
★★★★★ 4
Solid Budget Monitor Arm With a Few Trade‑Offs
Size: 13"-34", Style: Single Arm
The WALI Single Monitor Mount performs about the same as my VIVO single monitor arm, which was a pleasant surprise given the lower price point. Setup was straightforward, and the gas spring adjustment works well once dialed in. It holds my monitor securely and offers the full range of motion I expected—tilt, swivel, rotation, and height adjustments all function smoothly.
That said, the materials and overall build quality do feel a bit cheaper compared to higher‑end arms. It’s not flimsy, but you can tell where costs were saved. If you’re willing to spend roughly 60–70% more, you can definitely get a noticeably sturdier and more refined arm. Still, for a budget option, this one delivers exactly what it promises.
Pros
- Works just as well as the VIVO arm in terms of functionality
- Easy to install with both clamp and grommet options
- Gas spring provides good adjustability once set
- Excellent value for an entry‑level monitor arm
Cons
- Materials feel cheaper than premium alternatives
- Build quality isn’t as high as more expensive arms
Overall
As an introductory monitor arm, this is an excellent choice. It does everything the VIVO arm can, just with slightly lower‑end materials. If you’re on a budget or setting up a simple workstation, it’s a great fit. If you want something more robust for long‑term heavy use, spending a bit more will get you a significantly more refined Monitor arm.
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Reviewed in the United States on December 23, 2025
★★★★★ 5
Sturdy and easy to assemble
Size: 13"-34", Style: Single Arm, Size: 13"-34", Style: Single Arm
Really impressed with this monitor mount. The build quality feels solid and sturdy, especially for the price. It holds the monitor securely with no sagging or wobble, and the adjustability makes it super easy to get the perfect position. It’s been absolutely perfect for my sim racing setup being able to move the screen exactly where I want it made a huge difference in comfort and immersion. Definitely feels like a premium product without the premium price tag.
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Reviewed in the United States on May 16, 2026
★★★★★ 5
Reliable, affordable, and user-friendly
Size: 13"-34", Style: Single Arm, Size: 13"-34", Style: Single Arm
The installation process was straightforward and didn’t take much time at all—even if you’re not particularly handy, it’s easy to figure out.
What really stands out is how flexible the arm is. It adjusts smoothly in multiple directions, making it simple to position my monitor exactly where I want it. Whether I’m working, watching something, or multitasking, the range of motion makes a noticeable difference in comfort and productivity.
It’s also very sturdy once set up. My monitor feels secure, and I haven’t experienced any sagging or instability. As a second screen mount, it integrates perfectly with my desk setup and helps keep everything organized and clutter-free.
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Reviewed in the United States on April 17, 2026