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Description
Human LOX1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Lectin Like Oxidized Low Density Lipoprotein Receptor 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Lectin-like oxidized low-density lipoprotein receptor 1 (LOX1), also known as oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1), is a protein encoded by the OLR1 gene. LOX-1 is the primary receptor for oxidized low-density lipoproteins (OLDL) on endothelial cells, macrophages, smooth muscle cells, and other cell types. However, minimally oxidized LDL is more readily recognized by the TLR4 receptor, while highly oxidized LDL is more readily recognized by the CD36 receptor. LOX-1 is a receptor protein that belongs to the C-type lectin superfamily. Its gene expression is regulated by the cyclic AMP signaling pathway. This protein binds to, internalizes, and degrades OLDL. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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Product Reviews
★★★★★ 5
Comfortable women’s top, excellent for nursing!
Color: Black, Size: Large
I needed a new breastfeeding friendly tank top as I am postpartum and gained some weight. This is so comfortable and easily accessible for feeds. I wear it around the house and to sleep in. At a fraction of the cost of the more expensive brands nursing tops, this is a great practical, comfortable buy. The material is thick but breathable and not restrictive and non see through. Sizing is spot on. I am 5’4 and 160 lbs and I bought a Large. Overall this is a durable piece that is a staple in my postpartum wardrobe.
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Reviewed in the United States on April 11, 2026
★★★★★ 5
Great top for summer!
Color: Navy Blue, Size: Large
I bought one of these. The v-neck and fit of the tank top is amazing! Flattering! I loved it so much I ordered two more. Great summer tank! Washes and dries fabulously as well. I am 5’4” 150lbs, wear a medium or size 8-10. I purchased a large and love the look. It fits well, not too tight but hugs the body. Not oversized.
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Reviewed in the United States on May 23, 2026
★★★★★ 5
True to size & color, modest cut, fitted style
Color: Purple, Size: Small
The cut on these tops are perfect. It's a nice v-neck without being revealing. Thick straps and solid back so your bra doesn't show. Sizing is accurate in my case. 5'6" 130lbs chest 34" waist 26" hips 39" and I ordered the small which is fitted on me. I ordered the lavender purple and a black one and both colors are correct in shade. Soft and stretchy material. I air dry them mostly and only gentle low on the dryer if needed and have not had any shrinking.
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Reviewed in the United States on May 24, 2026
★★★★★ 5
Great Product
Size: 11.5-12.5 Women/10.5-11.5 Men, Color: Black-w001
This is a great water shoe, lightweight and very functional. Fits true to size. Very comfortable and love the no slip bottom. Well worth the purchase price
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Reviewed in the United States on April 29, 2026
★★★★★ 5
A Diabetic Traveler's Best Friend at the Hot Springs!
Size: 13-14 Women/12-13 Men, Color: Black-w001
I'll be honest — I bought these almost last minute before heading out on vacation to the Hot Springs in Carson City, Nevada. As someone with diabetes, I have to be really careful about my feet, especially on rough or hot concrete surfaces. Pool decks and spa areas can be brutal, and I've had too many close calls just walking around barefoot. I grabbed these water shoes almost as an afterthought, and they ended up being one of the best decisions of the whole trip.
From the moment I slipped them on, I could tell they were going to do the job. They fit true to size — no awkward sizing guesswork — and they hugged my feet snugly without feeling restrictive or causing any pressure points (a big deal for me). The sole gave me just enough protection from the concrete and wet surfaces around the springs, and I never once felt like I was going to slip. That peace of mind alone was worth every penny.
They're lightweight enough that I barely noticed I had them on, and they dried quickly after getting splashed or submerged. No heavy, waterlogged feeling dragging me down. I wore them in and out of the water all day and my feet felt totally fine — no soreness, no irritation, nothing. For someone managing diabetes, that's honestly huge.
I genuinely have zero complaints. These delivered exactly what they promised and then some. Whether you're headed to a hot spring, a beach, a pool, or just doing some light water yoga, these are a solid buy.
Hope this helps!
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Reviewed in the United States on May 14, 2026