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Absin 7-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)Product Specification References 1. Mingzhu Yang, et al., Chemical induced chromatin remodeling reprograms mouse ESCs to totipotent like stem cells. Cell Stem Cell 2022 Mar 3 Xin Guan, et al., Nanoparticle enhanced radiotherapy synergizes with PD L1 blockade to limit post surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 Qian Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and
Product Specification
| References | 1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell 2022 Mar 3 Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications Feb 9, 2022 4. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, September 2008. Ed Harlow, David Lane. Technical guidelines for antibodies (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. 7. Money helps the country. Jiao Lei. Application of multi-labeled immunofluorescence staining and Doppler imaging in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Usage | 1. Dilution of fluorescent dye: If the dye is dry powder, use 100ul DMSO to dissolve it to make 100X dye mother liquor; If the dye is liquid, it means that it is 100X mother liquor dissolved in DMSO from the factory; Use the signal amplification reaction solution to dilute at 1: 100 to prepare the dye working solution (ready-to-use); DAPI prepares the working solution using sterile water dilution at 1: 100. |
| Experimental Procedure | https://univ.oss-cn-shanghai.aliyuncs.com/absin-china/images/experiment/20190514165940524.png |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Synonym | Multicolor kit |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single index, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, and then the next primary antibody is replaced for the second round of incubation, and another fluorescein substrate is replaced, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 520, TSA monochromatic fluorescent dye 540, TSA monochromatic fluorescent dye 570, TSA monochromatic fluorescent dye 620, TSA monochromatic fluorescent dye 650, TSA monochromatic fluorescent dye 700, signal amplification reaction solution, pika universal HRP labeled secondary antibody, anti-fluorescence quenching encapsulation tablet, DAPI. |
| General Notes | 1. This kit is only used for immunohistochemistry and is not used for other purposes. 2. This kit is restricted to professional use only. 3. Appropriate protective measures should be taken to avoid contact of the reagent with the skin and eyes. 4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used. 5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process. 6. Incomplete dewaxing will easily affect the dyeing effect. 7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment. 8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management. |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2 ~ 8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell crawling sections, and needs to be matched with abs994 antibody eluate (specific for mIHC). |
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4.9 ★★★★★
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★★★★★ 5
Very good!
Format: Paperback
Book worth gold I love it! I will read it again! I recommend you buy it! Open your mind and make finances 🧠✨️
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Reviewed in the United States on April 1, 2026
★★★★★ 4
8.5 de 10
Format: Kindle
Lenguaje solo para profesionales y no para principiantes. Lo demás es perfecto con 4 estrellas ya que hasta lloré 3 veces ...
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Reviewed in the United States on April 22, 2026
★★★★★ 5
Interesting
Format: Paperback
Product with very good quality, its description matches the website.
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Reviewed in the United States on April 11, 2026
★★★★★ 5
A wonderful primer for those discovering the amazing world of generative AI
Format: Paperback, Format: Paperback
Somewhere in the past year, ChatGPT has gone from "cool, interesting, amusing" to a massively valuable work assistant capable of writing Python scripts, analysing data, and doing lots, lots, more. The key to this? The rapid evolution of OpenAI's GPT models and making my first forays into prompt engineering.
If I thought I was getting good, though, this book took me reminded me that I'm just scratching the surface. Halfway through the first chapter I was already furiously scribbling notes in the margins for what I could do better with my prompt writing and by the end of the text I felt like I had gotten a very good grounding - not just in GPTs specifically but in the bigger picture of how these hugely powerful tools were trained and came to maturity.
I imagine that few will argue with my assertion that there is lots of hyperbole and "noise" in the AI space right now which, as ever, makes it hard to pick out the signal from the noise. Which is precisely why I sought out an O'Reilly title and I'm very glad that I did.
Thorough, excellent, and I hope that this edition will be the first of many. As this rapidly maturing field scales and matures I think that prompt engineering will be an essential discipline to master. Pick up this text to get a good foothold on things.
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Reviewed in the United States on August 3, 2024
★★★★★ 4
Understanding AI prompts as a science
Format: Audiobook
Prompt engineering is the art and science of finding the right words to generate the right responses from artificial intelligence (AI). It's becoming a skill in-demand in today's workplaces. It's also becoming an essential skill for life. To understand prompt engineering, you must understand how AI tools understand their inputs. This book explains that to you in many forms.
It covers prompting as it intersects all the major AI disciplines, like general prompts (as in a ChatBot), fine-tuning, and retrieval-augmented generation (RAG). It also introduces how prompting intersects a couple of image-generation tools.
As a scientist, I appreciate the theoretical approach to these practical matters. Too many people are hacks at AI these days, and any technical understanding can rapidly advance an individual's effectiveness. Prompt engineering is often made fun of as a "soft" topic, but as this book demonstrates, it intersects all the major AI areas. Presumably less garbage in means less garbage out. This book shows how you can make that take place.
As typical for O'Reilly materials, this book appeals to people who desire an intermediate-to-advanced understanding of how AI works. It's not for the casual user. Anyone technical involved in professional knowledge generation using AI can benefit from understanding the dynamics "under the hood." It's a good, though perhaps not ground-breaking, textbook for those of us unable to take a class in the subject. Prompt engineering may be a soft topic to many, but books like this ground the field in the science that can make or break a digital or software product.
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Reviewed in the United States on December 13, 2025