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Description
Human HSL ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed immediately or stored at -20°C or -80°C, but avoid repeated freeze-thaw cycles. 3. Pre-Assay Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Hormone Sensitive Lipase (HSL) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Hormone Sensitive Lipase (HSL) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Hormone Sensitive Lipase ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Hormone-sensitive lipase (HSL), formerly known as cholesterol ester hydrolase (CEH) and sometimes referred to as triacylglycerol lipase, is an enzyme encoded by the LIPE gene. HSL is an intracellular neutral lipase capable of hydrolyzing a variety of esters. The enzyme exists in two forms: a long and a short. The long form is expressed in steroidogenic tissues, such as the testes, where it converts cholesterol esters into free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, where it, among other things, hydrolyzes stored triglycerides into free fatty acids. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma |
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4.4 ★★★★★
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Product Reviews
★★★★★ 5
This is a very useful field manual for those interested in detailed traking methodology.
Format: Paperback
I spent many years as interpreter and ranger for the Colorado State Park System, now called CPW—Colorado Parks and Wildlife. We were annually tasked with surveying a given species' presence, density, and range in the park system and surrounding areas. Detailed field manuals were critical to the accuracy of our work, and HOW I WISH I HAD THIS BOOK DURING THAT PART OF MY CAREER. There are few other books I've come across providing detailed, yet very accessible information on how trail sign reflects animal behavior across different conditions, landscapes, and seasons (both weather seasons and mating seasons). One particular aspect of this book I found significantly intriguing was the section on predation—how does a mountain lion take down a mule deer vs. how wolves bring one down. The locations on a prey animal where a certain predator is most likely to attack, showing illustrations, is a remarkable piece of work, and there authors are clearly masters of interpreting tracks and sign! If you want learn about how mammals behave in their native environment, adding this book to your field manual packet will greatly expand your horizons! Busy it, read it, and get outside! Thank you Mr. Elbroch and Mr. McFarland for adding to the wildlife canon!
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Reviewed in the United States on August 5, 2023
★★★★★ 5
Great book and very useful tool to have
Format: Paperback
I had the older edition and lived it, but someone at my last job *borrowed* it and I never saw it again. This edition sends even better than the last, and I have that one five stars. Great pictures and the number of species covered means it's good in at least all states I've worked in.
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Reviewed in the United States on November 16, 2025
★★★★★ 5
Simply unputdownable
Format: Kindle
B N William’s field guide is fascinating. The book uncovers the bizarre reality beneath the polished propaganda of the Second World War, exploring the wonderfully weird truths where the line between brilliant innovation and sheer insanity vanishes completely. Every unusual piece of information has been verified using official declassified archives. I loved the story of Wojtek, a Syrian brown bear, who became a beloved and legendary figure, known for his unique story as an enlisted soldier. Read many more such stories and episodes in this book. You will enjoy them thoroughly.
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Reviewed in the United States on November 25, 2025
★★★★★ 5
Excellent read
Format: Kindle
This book is a deep dive into the truths behind the war we think we know. The book talks absurd strategies, eccentric leaders, and bizarre battlefield moments that genuinely happened. Each fact is both shocking and fascinating. it's entertaining and wildly surprising read. It’s a perfect pick for history buffs, trivia lovers.
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Reviewed in the United States on November 24, 2025
★★★★★ 5
Wonderful reading
Format: Paperback
It is a pleasure to “listen” to MLK Jr, and his description of the Montgomery boycott, mostly after reading the newest biography King, a life. Both books are highly recommended.
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Reviewed in the United States on April 20, 2026
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