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Description
Human OXTR ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an oxytocin receptor (OXTR) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of oxytocin receptor (OXTR) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Oxytocin Receptor ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | The oxytocin receptor (OXTR) is a protein that functions as a receptor for the hormone and neurotransmitter oxytocin. The OXTR protein belongs to the G protein-coupled receptor family, specifically Gq, and acts as a receptor for oxytocin. Its activity is mediated by G proteins, activating several different second messenger systems. It is expressed by the myoepithelial cells of the mammary gland and in the myometrium and endometrium during late pregnancy. It is also involved in the central nervous system. The gene is thought to play an important role in social, cognitive, and emotional behavior. Increased expression of the gene is thought to be associated with unemotional traits, rigid thinking, and problems recognizing faces and emotions. Decreased expression of the gene is thought to contribute to prenatal stress, postpartum depression, and social anxiety. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.6 ★★★★★
Based on 1682 reviews
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Product Reviews
★★★★★ 5
Better than the movie
Format: Kindle
I really loved this book. The Disney movie was clearly only very loosely based on this wonderful story. I’m actually kinda mad I ever saw the movie because it kept me from the book for so long.
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Reviewed in the United States on December 16, 2025
★★★★★ 5
A fairytale classic made even better!
Format: Paperback
Embellishing on the original tale of Cinderella, Gail Levine has written an imaginative and touching story - a classic in its own right. While the traditional fairytale focuses on the magical rags-to-riches transformation of a scullery maid into a princess, Levine's heroine is very real in both her defeats and her triumphs. Ella is a wonderful character whose unwavering sense of humor and refusal to succumb make her an endearing heroine whom the reader eagerly follows through her journeys. The friendship that she shares with Prince Char, her struggles to break the curse placed on her at birth, the pain and the joy that she feels at each turn of events are drawn with a clarity and poignant charm that make this a unique story all its own. There is, of course, glass slippers, a ball, the pumpkin coach - but this story goes far beyond the original to become something more. It is not just about one magical night that makes a servant into a princess; it is about the life of a girl who is more than what she seems and has only to realize it for herself to make her wishes come true.
Ella Enchanted is a wonderful story for readers of all ages, and there is an amazing depth to this tale that cannot help but make you think. Despite the omnipresent existence of magic and the whimsical creatures that include everything from gnomes and elves to giants and ogres, the focus of the story is very real. We are, each of us, a little bit like Ella - struggling to be who we are while everything around us tries to make us otherwise. I strongly recommend this book to anyone who loves fairytales and children's books - especially to anyone who loves the story of Cinderella.
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Reviewed in the United States on July 18, 2000
★★★★★ 5
Timeless
Format: Hardcover
My first born daughter is literally named Ella after Ella Enchanted. This is the most meaningful children’s book I ever experienced in childhood and it continues to hold up as I have reread it to my Ella in parenthood. It’s still so fun and magical and heartfelt. Thank you Gail Carson Levine for this literary gem!
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Reviewed in the United States on June 25, 2025
★★★★★ 4
Beloved childhood classic for a reason
Format: Paperback
Not to give away my age, but I owned this book when I was younger and lent it to a friend and never got it back. I finally gave into my desire to own it again and purchased it from amazon. I'm so happy that I did. This book definitely holds up even after all these years. It's this kind of writing that helped me fall in love with the idea of being a writer. I've read some of Gail Carson Levine's other books and enjoyed them to varying degrees. I remember reading The Wish and thinking, even as a child, that it was incredibly bland. But Ella Enchanted is truly one of her gems.
It is heartfelt and well-written with a truly wonderful heroine. Now that I'm older I do see more of the problems. The writing is a lot simpler than I remember but I suppose that it needs to be for the reading level of the target audience so I won't count that as a negative. As with most young adult novels, it is on the shorter side. This leads to some awkward pacing and a lack of development. There's not really enough of her friend Areida or her adventures. The romance with Char is underdeveloped though conveyed skillfully enough that by the end I was very invested in the outcome. The ending and epilogue after the dramatic conclusion also come across as a little rushed. Still, I think it's a wonderful story for young girls and a truly inspiring heroine for them to identify with. For older readers, it's a pleasant diversion. The strength of the story is Ella and the way Levine writes the scenes that are meant to have a big emotional impact. I understand when people say the story meanders a bit and how it could be boring for some people. Levine doesn't do a great job with descriptions despite the complexity of the world she's imagined. It's difficult to really picture anything in your head based on the descriptions she gives. She's not a world-builder like the big name fantasy writers. She's more interested in the characters and the main plot arc for the heroine.
I would recommend this book for a reader or parent looking for a strong, believable female protagonist. It's not a great fantasy novel and it's a little underdeveloped but the individual scenes are well written and the emotion, lesson, and strong character arc are there. I'd also recommend getting the new version as there are great extras including information about the languages Levine developed for the various creatures in the world of Ella Enchanted. I don't remember these being included in the first edition.
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Reviewed in the United States on July 20, 2012
★★★★★ 5
Little golden book Hawkeye
Format: Hardcover
A nice marcel book to keep
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Reviewed in the United States on January 1, 2026